Low DNA Taq HS, 5 U/µL is a highly purified chemically modified hot start Taq DNA polymerase and buffer system, with separate magnesium. It has been developed for low bioburden and very high sensitivity over a wide range of PCR templates.
A chemical moiety is attached to Low DNA Taq HS, 5 U/µL making it inactive at ambient temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. Low DNA Taq HS, 5 U/µL catalyses 5′-3′ synthesis and possesses low 5′-3′ exonuclease activity, however these are not detectable before activation, enhancing PCR sensitivity. The functional activity of the enzyme is restored during a 10-minute incubation at 95°C. The low DNA background and stringent hot-start properties of Low DNA Taq HS, 5 U/µL are ideal for PCR of low-copy bacterial targets and avoiding false-positive amplification, such as in water testing.
- Chemical hot start Taq polymerase
- Reduced effects of primer dimerisation or mispriming that causes PCR nonspecific bands
- Low bioburden improves low-copy target amplification
- Convenient room temperature PCR set-up
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